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Image Search Results
Journal: bioRxiv
Article Title: Escherichia coli’s physiology can turn membrane voltage dyes into actuators
doi: 10.1101/607838
Figure Lengend Snippet: E. coli growth in the presence of ThT. E. coli growing in MM9 media supplemented with glucose or (B) glycerol at increasing ThT concentration (colourmap). ThT concentrations in (A) are 10, 25, 50, 75, 100, 200, 350 µ M and in (B) 10, 25, 50, 75, 100, 150, 175, 200 µ M). The error bars are standard deviations. (C) Maximum growth rates from (A) and (B) for each ThT concentration are given in red and blue respectively. Each condition was done at least in triplicate and error bars are the standard deviation. (D) I in against time in LB and in (E) MM9 + glucose media. Individual cells are shown in cyan (45 in (D) and 52 in (E) from at least 9 independent experiments), and the average trace is shown in blue. The imaging conditions and I ex = 10 µM are the same for (E) and (D).
Article Snippet: Upon harvest we washed the cells into fresh LB or
Techniques: Concentration Assay, Standard Deviation, Imaging
Journal: bioRxiv
Article Title: Escherichia coli’s physiology can turn membrane voltage dyes into actuators
doi: 10.1101/607838
Figure Lengend Snippet: Comparison of WT and ΔTolC mutant response to ThT. (A) I in versus time for the WT (cyan) and ΔTolC (gray) loaded in LB. WT traces are reproduced from and ΔTolC traces were obtained from 5 independent experiments to give 23 single cell traces. Averaged traces for the WT and ΔTolC are given in blue and black respectively. (B) Growth curves of WT and ΔTolC in MM9 + glucose media are given in blue (reproduced from ) and black, respectively. Growth curves in the same media, but in the presence of 10 µ M ThT, are given in cyan (WT) and gray (ΔTolC). The shaded areas show standard deviation and cyan and blue growth curves for the WT overlap.
Article Snippet: Upon harvest we washed the cells into fresh LB or
Techniques: Mutagenesis, Standard Deviation